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Sequenom sequenom massarray methylation analysis
Sequenom Massarray Methylation Analysis, supplied by Sequenom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/massarray+analysis/analysis+massarray+methylation+sequenom/pm40999405-144-13-13
Average 86 stars, based on 1 article reviews
sequenom massarray methylation analysis - by Bioz Stars, 2026-10
86/100 stars

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Methylation:

Article Title: miR194 hypomethylation regulates coronary artery disease pathogenesis
Article Snippet: In 2018, the Infinium HumanMethylation450 assay was used to examine the genome-wide DNA methylation profiles in three pairs of samples from patients with acute coronary syndrome and control samples. .. Methylation-specific polymerase chain reaction (MSP) was also used to validate Sequenom MassARRAY analysis in ACS (acute coronary syndrome), stable coronary artery disease, and control samples. ..

Article Title: Regulatory Network of Two Tumor-Suppressive Noncoding RNAs Interferes with the Growth and Metastasis of Renal Cell Carcinoma
Article Snippet: .. *p < 0.05. (E) Aberrant methylation in MEG3 promoter determined by Sequenom MassARRAY analysis in three matched RCC and adjacent normal tissues, as well as two cell lines ACHN and 786-O. ..

Article Title: Hepatitis B Virus Demethylates PD-1 and Modulates the Changes of the Tumor Microenvironment in Hepatocellular Carcinoma Patients
Article Snippet: .. Detection the expression of PD-1 on CD8+ T cells by Flow cytometric analysis in pCS-HBV1.3 group and pCS-CG group; B. Sequenom MassARRAY analysis of PD-1 gene methylation in pCS-HBV1.3 group and pCS-CG group; C. Cytokine measurement in pCS-HBV1.3 group and pCS-CG group. ..

Polymerase Chain Reaction:

Article Title: miR194 hypomethylation regulates coronary artery disease pathogenesis
Article Snippet: In 2018, the Infinium HumanMethylation450 assay was used to examine the genome-wide DNA methylation profiles in three pairs of samples from patients with acute coronary syndrome and control samples. .. Methylation-specific polymerase chain reaction (MSP) was also used to validate Sequenom MassARRAY analysis in ACS (acute coronary syndrome), stable coronary artery disease, and control samples. ..

Control:

Article Title: miR194 hypomethylation regulates coronary artery disease pathogenesis
Article Snippet: In 2018, the Infinium HumanMethylation450 assay was used to examine the genome-wide DNA methylation profiles in three pairs of samples from patients with acute coronary syndrome and control samples. .. Methylation-specific polymerase chain reaction (MSP) was also used to validate Sequenom MassARRAY analysis in ACS (acute coronary syndrome), stable coronary artery disease, and control samples. ..

Article Title: SLCO3A1 , a Novel Crohn’s Disease-Associated Gene, Regulates NF-κB Activity and Associates with Intestinal Perforation
Article Snippet: SNPs from 16 CD patients and 16 age- and sex-matched control patients were analyzed using Illumina platform analysis. .. Subsequently, we expanded the study and followed 53 CD patients and 41 control patients by Sequenom MassArray analysis. ..

Biomarker Discovery:

Article Title: SNP markers tightly linked to root knot nematode resistance in grapevine ( Vitis cinerea ) identified by a genotyping-by-sequencing approach followed by Sequenom MassARRAY validation
Article Snippet: .. However, validation of SNPs using Sequenom MassARRAY analysis indicated that the GBS pipeline results do not easily transfer with only 50% of the SNPs producing useful markers at the MJR1 locus. ..

Comparison:

Article Title: Establishment and Characterisation by Expression Microarray of Patient-Derived Xenograft Panel of Human Pancreatic Adenocarcinoma Patients
Article Snippet: .. Supplementary Materials can be found at https://www.mdpi.com/1422-0067/21/3/962/s1 ; Figure S1: Representative H&E images of patient tumours in comparison to corresponding PDX F1 tumours of PIN 065 (A & B), PIN 080 (C & D), PIN 089 (E & F) PIN 091 (G & H), PIN 099 (I & J); Figure S2: Mutations detected by Sequenom MassArray Analysis in 10 PDX F1 tumour samples; Table S1: Gene and mutations detected in 10 F1 PDX samples by Sequenom MassArray Analysis; Table S2: Shortlist of selected 89 genes increased in T compared to N and also in F1 compared to T; Table S3: List of mutations analysed using the Agena MassArray technology. ..

Expressing:

Article Title: Hepatitis B Virus Demethylates PD-1 and Modulates the Changes of the Tumor Microenvironment in Hepatocellular Carcinoma Patients
Article Snippet: .. Detection the expression of PD-1 on CD8+ T cells by Flow cytometric analysis in pCS-HBV1.3 group and pCS-CG group; B. Sequenom MassARRAY analysis of PD-1 gene methylation in pCS-HBV1.3 group and pCS-CG group; C. Cytokine measurement in pCS-HBV1.3 group and pCS-CG group. ..



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Expression of CTHRC1 correlates with DNA <t>methylation</t> levels in HCC cell lines. A The methylation levels of CTHRC1 in the promoter region were detected by Agena <t>MassARRAY®</t> Methylation in HSC cell line LX-2 and three HCC cell lines HepG2, Huh-7, and Hep3B2.1 (left), and presented in hot map (right). B RT-qPCR and C western bolt were used to detect the mRNA and protein levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. LX-2 group. D RT-qPCR was used to detect the mRNA levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines treated with 5-Aza-DC. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
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Expression of CTHRC1 correlates with DNA <t>methylation</t> levels in HCC cell lines. A The methylation levels of CTHRC1 in the promoter region were detected by Agena <t>MassARRAY®</t> Methylation in HSC cell line LX-2 and three HCC cell lines HepG2, Huh-7, and Hep3B2.1 (left), and presented in hot map (right). B RT-qPCR and C western bolt were used to detect the mRNA and protein levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. LX-2 group. D RT-qPCR was used to detect the mRNA levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines treated with 5-Aza-DC. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
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Expression of CTHRC1 correlates with DNA <t>methylation</t> levels in HCC cell lines. A The methylation levels of CTHRC1 in the promoter region were detected by Agena <t>MassARRAY®</t> Methylation in HSC cell line LX-2 and three HCC cell lines HepG2, Huh-7, and Hep3B2.1 (left), and presented in hot map (right). B RT-qPCR and C western bolt were used to detect the mRNA and protein levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. LX-2 group. D RT-qPCR was used to detect the mRNA levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines treated with 5-Aza-DC. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
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Expression of CTHRC1 correlates with DNA <t>methylation</t> levels in HCC cell lines. A The methylation levels of CTHRC1 in the promoter region were detected by Agena <t>MassARRAY®</t> Methylation in HSC cell line LX-2 and three HCC cell lines HepG2, Huh-7, and Hep3B2.1 (left), and presented in hot map (right). B RT-qPCR and C western bolt were used to detect the mRNA and protein levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. LX-2 group. D RT-qPCR was used to detect the mRNA levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines treated with 5-Aza-DC. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
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Expression of CTHRC1 correlates with DNA <t>methylation</t> levels in HCC cell lines. A The methylation levels of CTHRC1 in the promoter region were detected by Agena <t>MassARRAY®</t> Methylation in HSC cell line LX-2 and three HCC cell lines HepG2, Huh-7, and Hep3B2.1 (left), and presented in hot map (right). B RT-qPCR and C western bolt were used to detect the mRNA and protein levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. LX-2 group. D RT-qPCR was used to detect the mRNA levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines treated with 5-Aza-DC. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
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Expression of CTHRC1 correlates with DNA methylation levels in HCC cell lines. A The methylation levels of CTHRC1 in the promoter region were detected by Agena MassARRAY® Methylation in HSC cell line LX-2 and three HCC cell lines HepG2, Huh-7, and Hep3B2.1 (left), and presented in hot map (right). B RT-qPCR and C western bolt were used to detect the mRNA and protein levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. LX-2 group. D RT-qPCR was used to detect the mRNA levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines treated with 5-Aza-DC. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Journal: Discover Oncology

Article Title: CTHRC1 modulates cell proliferation and invasion in hepatocellular carcinoma by DNA methylation

doi: 10.1007/s12672-024-01194-8

Figure Lengend Snippet: Expression of CTHRC1 correlates with DNA methylation levels in HCC cell lines. A The methylation levels of CTHRC1 in the promoter region were detected by Agena MassARRAY® Methylation in HSC cell line LX-2 and three HCC cell lines HepG2, Huh-7, and Hep3B2.1 (left), and presented in hot map (right). B RT-qPCR and C western bolt were used to detect the mRNA and protein levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. LX-2 group. D RT-qPCR was used to detect the mRNA levels of CTHRC1 in LX-2, HepG2, Huh-7, and Hep3B2.1 cell lines treated with 5-Aza-DC. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Agena MassARRAY® Methylation Analysis assessed the methylation level of CTHRC1 in the promoter region.

Techniques: Expressing, DNA Methylation Assay, Methylation, Quantitative RT-PCR, Western Blot